The importance of Indigenous and local people for cataloging biodiversity.

Indigenous and local peoples' (ILPs) role in cataloging life on Earth has been significant but underappreciated. ILPs knowledge faces growing cultural and biological threats. Greater participation by ILPs in research would make science more efficient, conservation more sustainable, and traditional knowledge stronger, but formidable obstacles remain.

Biocultural vulnerability exposes threats of culturally important species.

There are growing calls for conservation frameworks that, rather than breaking the relations between people and other parts of nature, capture place-based relationships that have supported social-ecological systems over the long term. Biocultural approaches propose actions based on biological conservation priorities and cultural values aligned with local priorities, but mechanisms that allow their global uptake are missing. We propose a framework to globally assess the biocultural status of specific components of nature that matter to people and apply it to culturally important species (CIS). Drawing on a literature review and a survey, we identified 385 wild species, mostly plants, which are culturally important. CIS predominate among Indigenous peoples (57%) and ethnic groups (21%). CIS have a larger proportion of Data-Deficient species (41%) than the full set of International Union for Conservation of Nature (IUCN) species (12%), underscoring the disregard of cultural considerations in biological research. Combining information on CIS biological conservation status (IUCN threatened status) and cultural status (language vitality), we found that more CIS are culturally Vulnerable or Endangered than they are biologically and that there is a higher share of bioculturally Endangered or Vulnerable CIS than of either biologically or culturally Endangered CIS measured separately. Bioculturally Endangered or Vulnerable CIS are particularly predominant among Indigenous peoples, arguably because of the high levels of cultural loss among them. The deliberate connection between biological and cultural values, as developed in our "biocultural status" metric, provides an actionable way to guide decisions and operationalize global actions oriented to enhance place-based practices with demonstrated long-term sustainability.

Language extinction triggers the loss of unique medicinal knowledge.

Over 30% of the 7,400 languages in the world will no longer be spoken by the end of the century. So far, however, our understanding of whether language extinction may result in the loss of linguistically unique knowledge remains limited. Here, we ask to what degree indigenous knowledge of medicinal plants is associated with individual languages and quantify how much indigenous knowledge may vanish as languages and plants go extinct. Focusing on three regions that have a high biocultural diversity, we show that over 75% of all 12,495 medicinal plant services are linguistically unique-i.e., only known to one language. Whereas most plant species associated with linguistically unique knowledge are not threatened, most languages that report linguistically unique knowledge are. Our finding of high uniqueness in indigenous knowledge and strong coupling with threatened languages suggests that language loss will be even more critical to the extinction of medicinal knowledge than biodiversity loss.

New Guinea has the world's richest island flora.

New Guinea is the world's largest tropical island and has fascinated naturalists for centuries1,2. Home to some of the best-preserved ecosystems on the planet3 and to intact ecological gradients-from mangroves to tropical alpine grasslands-that are unmatched in the Asia-Pacific region4,5, it is a globally recognized centre of biological and cultural diversity6,7. So far, however, there has been no attempt to critically catalogue the entire vascular plant diversity of New Guinea. Here we present the first, to our knowledge, expert-verified checklist of the vascular plants of mainland New Guinea and surrounding islands. Our publicly available checklist includes 13,634 species (68% endemic), 1,742 genera and 264 families-suggesting that New Guinea is the most floristically diverse island in the world. Expert knowledge is essential for building checklists in the digital era: reliance on online taxonomic resources alone would have inflated species counts by 22%. Species discovery shows no sign of levelling off, and we discuss steps to accelerate botanical research in the 'Last Unknown'8.

Reconstructing the Complex Evolutionary History of the Papuasian Schefflera Radiation Through Herbariomics.

With its large proportion of endemic taxa, complex geological past, and location at the confluence of the highly diverse Malesian and Australian floristic regions, Papuasia - the floristic region comprising the Bismarck Archipelago, New Guinea, and the Solomon Islands - represents an ideal natural experiment in plant biogeography. However, scattered knowledge of its flora and limited representation in herbaria have hindered our understanding of the drivers of its diversity. Focusing on the woody angiosperm genus Schefflera (Araliaceae), we ask whether its morphologically defined infrageneric groupings are monophyletic, when these lineages diverged, and where (within Papuasia or elsewhere) they diversified. To address these questions, we use a high-throughput sequencing approach (Hyb-Seq) which combines target capture (with an angiosperm-wide bait kit targeting 353 single-copy nuclear loci) and genome shotgun sequencing (which allows retrieval of regions in high-copy number, e.g., organellar DNA) of historical herbarium collections. To reconstruct the evolutionary history of the genus we reconstruct molecular phylogenies with Bayesian inference, maximum likelihood, and pseudo-coalescent approaches, and co-estimate divergence times and ancestral areas in a Bayesian framework. We find strong support for most infrageneric morphological groupings, as currently circumscribed, and we show the efficacy of the Angiosperms-353 probe kit in resolving both deep and shallow phylogenetic relationships. We infer a sequence of colonization to explain the present-day distribution of Schefflera in Papuasia: from the Sunda Shelf, Schefflera arrived to the Woodlark plate (present-day eastern New Guinea) in the late Oligocene (when most of New Guinea was submerged) and, subsequently (throughout the Miocene), it migrated westwards (to the Maoke and Bird's Head Plates and thereon) and further diversified, in agreement with previous reconstructions.

Relación entre la composición florística y los nutrientes del suelo en comunidades de palmas del Chocó biogeográfico en Colombia y Ecuador

En ecología una de las preguntas más importantes es: ¿cuáles son los factores ambientales que explican la alta diversidad de especies de plantas en los bosques tropicales? En este trabajo, se estudiaron las comunidades de palmas y su relación con los nutrientes del suelo en dos localidades del Chocó biogeográfico. Específicamente, se investigó: (1) cómo varían los nutrientes del suelo entre las dos localidades; (2) cuál es la relación entre los nutrientes del suelo y la composición florística de palmas; (3) cuáles son los nutrientes más importantes que explican la variación en la composición florística de las comunidades de palmas; y (4) cómo varía la abundancia de las especies dominantes de sotobosque y de dosel a lo largo del gradiente de concentración de los nutrientes más importantes. Se realizaron 20 transectos de 500 x 5 m (5 ha), en los cuales se identificaron y contaron todos los individuos de palmas en sus diferentes estadios de crecimiento. Las muestras de suelo se tomaron en cada uno de los transectos a los 0 , 250 y 500 m. Los cationes (Al, Ca, Mg, K, P) se extrajeron con la técnica Mehlich-III y las concentraciones se analizaron con espectrometría de emisión con plasma de acoplamiento inductivo (ICP-OES). La concentración de macronutrientes se relacionó con la composición florística mediante el test de Mantel, el test parcial de Mantel, regresiones lineales y escalamiento multidimensional no métrico (NMDS). Se encontraron 9 158 individuos y 33 especies de palmas en las 5 ha cubiertas por los transectos. La composición florística presentó una correlación positiva y significativa con los nutrientes del suelo (rM = 0.63-0.69) y con la distancia geográfica entre los transectos (rM = 0.71-0.75), mientras que las variables edáficas mostraron una alta correlación con la distancia geográfica (rM = 0.72). Asimismo, se encontró una débil relación significativa entre la fertilidad del suelo y la abundancia de palmas de sotobosque, pero ninguna con las palmas del dosel. Nuestros resultados demuestran que los suelos juegan un papel importante en la composición de las comunidades de palmas a escalas locales en la región del Chocó, y que los suelos más pobres y ricos en fósforo presentan la mayor diversidad de palmas.

One of the most important questions in ecology is: which are the environmental factors that explain the high plant diversity of tropical forests? We studied the floristic composition of palm communities and their relationship to soil nutrients in two localities of the Chocó region to understand (1) how soil nutrients vary between the two localities; (2) what is the relationship between soil nutrients and palm floristic composition; (3) what are the most important nutrients that explain the variation in floristic composition of palm communities; and (4) how does the abundance of the most exuberant understory and canopy palm species vary along the concentration gradient of the most important nutrients? We established 20 transects (5 x 500 m) and identified all palm individuals of all growth stages. Soil samples were taken at each transect at the beginning, middle, and end. Macronutrients (Al, Ca, Mg, K, P) were extracted with the Mehlich-III technique and their concentrations were analyzed by inductively coupled plasma emission spectrometry (ICP-OES). We used Mantel test, partial Mantel tests, linear regressions, and non-metric multidimensional scaling to determine if the concentration of nutrients was related to the floristic composition. We found a total of 9 158 individuals and 33 species of palms in the 5 ha covered by our transects. Floristic composition had a positive and significant correlation with soil nutrients (rM = 0.63-0.69) and with geographical distance between transects (rM= 0.71-0.75), whereas the soil nutrients were highly correlated with geographical distance (rM = 0.72). We also found a small, yet significant, relationship between soil fertility and the abundance of understory palms, but no relationship with canopy palms. Our results demonstrate for the first time that soils play an important role in the composition of palm communities at local scales in the Chocó region, and that infertile and phosphorus-rich soils have the highest palm diversity.

Indigenous knowledge networks in the face of global change.

Indigenous communities rely extensively on plants for food, shelter, and medicine. It is still unknown, however, to what degree their survival is jeopardized by the loss of either plant species or knowledge about their services. To fill this gap, here we introduce indigenous knowledge networks describing the wisdom of indigenous people on plant species and the services they provide. Our results across 57 Neotropical communities show that cultural heritage is as important as plants for preserving indigenous knowledge both locally and regionally. Indeed, knowledge networks collapse as fast when plant species are driven extinct as when cultural diffusion, either within or among communities, is lost. But it is the joint loss of plant species and knowledge that erodes these networks at a much higher rate. Our findings pave the road toward integrative policies that recognize more explicitly the inseparable links between cultural and biological heritage.

Tropical ulcer plant treatments used by Papua New Guinea's Apsokok nomads.

ETHNOPHARMACOLOGICAL RELEVANCE: The tropical ulcer is a debilitating bacterial infection that is common in Papua New Guinea. Deploying healthcare infrastructure to remote and inaccessible rainforest locations is not practical, therefore local plants may be the best treatment option. Here we present an ethnobotanical survey of the tropical ulcer plant medicines used by the semi-nomadic Apsokok who roam the remote central mountains of Papua New Guinea's West New Britain Province. In vitro biological activity in assays relevant to tropical ulcer wound healing is also presented. MATERIALS AND METHODS: Focus groups and semi-structured interviews were used to acquire information on the uses of plants, vouchers of which were identified by comparison with authentic herbarium specimens. Antibacterial disc diffusion assays with Staphylococcus aureus and Fusobacterium ulcerans, MMP-9 enzyme inhibition and dermal fibroblast stimulation assays were carried out on plant saps and aqueous extracts of plant material. LC-MS was used to identify known plant metabolites. RESULTS: The ethnobotanical survey identified sixteen species that were used to treat tropical ulcers, all of which were applied topically. A subset of twelve species were investigated further in vitro. Four species produced zones of inhibition with S. aureus, all 12 species provided low level inhibition of MMP-9 and 8 species stimulated dermal fibroblast proliferation, although cytotoxicity occurred at higher concentrations. The extract of Homalium foetidum Benth. inhibited S. aureus and MMP-9 while at lower sub-cytotoxic concentrations stimulated fibroblast proliferation. Trans-3-O-p-coumaroylquinic acid cis-3-O-p-coumaroylquinic acid were detected in the aqueous extract of H. foetidum. CONCLUSIONS: Topical application of plant saps to wounds results in very high localised concentrations of plant metabolites which is likely to result in inhibition of MMP proteases. H. foetidum is a candidate plant for tropical ulcer treatment in remote areas.

Fundamental species traits explain provisioning services of tropical American palms.

The well-being of the global human population rests on provisioning services delivered by 12% of the Earth's ∼400,000 plant species1. Plant utilization by humans is influenced by species traits2-4, but it is not well understood which traits underpin different human needs5. Here, we focus on palms (Arecaceae), one of the most economically important plant groups globally6, and demonstrate that provisioning services related to basic needs, such as food and medicine, show a strong link to fundamental functional and geographic traits. We integrate data from 2,201 interviews on plant utilization from three biomes in South America-spanning 68 communities, 43 ethnic groups and 2,221 plant uses-with a dataset of 4 traits (leaf length, stem volume, fruit volume, geographic range size) and a species-level phylogeny7. For all 208 palm species occurring in our study area, we test for relations between their traits and perceived value. We find that people preferentially use large, widespread species rather than small, narrow-ranged species, and that different traits are linked to different uses. Further, plant size and geographic range size are stronger predictors of ecosystem service realization for palm services related to basic human needs than less-basic needs (for example, ritual). These findings suggest that reliance on plant size and availability may have prevented our optimal realization of wild-plant services, since ecologically rare yet functionally important (for example, chemically) clades may have been overlooked. Beyond expanding our understanding of how local people use biodiversity in mega-diverse regions, our trait- and phylogeny-based approach helps to understand the processes that underpin ecosystem service realization, a necessary step to meet societal needs in a changing world with a growing human population5,8.

Geospatial patterns in traditional knowledge serve in assessing intellectual property rights and benefit-sharing in northwest South America.

ETHNOPHARMACOLOGICAL RELEVANCE: Without an understanding of the geography of traditional knowledge, implementing the Nagoya Protocol and national or regional strategies for benefit-sharing with local and indigenous communities will be difficult. We evaluate how much traditional knowledge about medicinal palm (Arecaceae) uses is unique and how much is shared across (i) four countries (Colombia, Ecuador, Peru, Bolivia), (ii) two cultural groups (Amerindian and non-Amerindian), (iii) 52 Amerindian tribes, (iv) six non-Amerindian groups, (v) 41 communities, and (vi) individuals in the 41 communities. MATERIALS AND METHODS: We first sampled traditional knowledge about palms from 255 references and then carried out 2201 field interviews using a standard protocol. Using the combined data set, we quantified the number of "singletons" that were unique to one of the analyzed scales. For the 41 communities, we evaluated how many uses were cited by <10% and by ≥50% of informants. We performed a Kruskal-Wallis test to evaluate whether the number of unshared uses (cited by <10%) differed significantly in relation to the informants׳ gender and degree of expertise, and performed a two-way ANOVA to test for differences in the number of unshared and shared uses accounted for by the five birth cohorts. RESULTS: We found that most knowledge was not shared among countries, cultural groups, tribes, communities, or even individuals within them. Still, a minor knowledge component was widely shared, even across countries. General informants cited a significantly higher number of unshared uses than experts, whereas no significant differences were found in the number of unshared uses cited by men and women or by different age groups. CONCLUSION: Our region-wide analysis highlights the geospatial complexity in traditional knowledge patterns, underscoring the need for improved geographic insight into the ownership of traditional knowledge in areas where biocultural diversity is high. This high geographic complexity needs consideration when designing property right protocols, and calls for countrywide compilation efforts as much localized knowledge remains unrecorded.

New categories for traditional medicine in the Economic Botany Data Collection Standard.

The Economic Botany Data Collection Standard (EBDCS) has been successfully followed by ethnobotanists investigating plant uses in many parts of the world. However, we have encountered some cases in our study of traditional medicine where the standard seems incomplete and inaccurate when it is applied to plant uses of rural or indigenous societies in developing countries. We propose two categories to be added to the EBDCS: Cultural Diseases and Disorders, and Ritual/Magical Uses. Adding these categories, we believe will give a more accurate insight into traditional medicine and will contribute to developing an integrative ethnomedicinal data collection protocol, which will make ethnomedicinal studies more comparable.

Ethnobotanical knowledge is vastly under-documented in northwestern South America.

A main objective of ethnobotany is to document traditional knowledge about plants before it disappears. However, little is known about the coverage of past ethnobotanical studies and thus about how well the existing literature covers the overall traditional knowledge of different human groups. To bridge this gap, we investigated ethnobotanical data-collecting efforts across four countries (Colombia, Ecuador, Peru, Bolivia), three ecoregions (Amazon, Andes, Chocó), and several human groups (including Amerindians, mestizos, and Afro-Americans). We used palms (Arecaceae) as our model group because of their usefulness and pervasiveness in the ethnobotanical literature. We carried out a large number of field interviews (n = 2201) to determine the coverage and quality of palm ethnobotanical data in the existing ethnobotanical literature (n = 255) published over the past 60 years. In our fieldwork in 68 communities, we collected 87,886 use reports and documented 2262 different palm uses and 140 useful palm species. We demonstrate that traditional knowledge on palm uses is vastly under-documented across ecoregions, countries, and human groups. We suggest that the use of standardized data-collecting protocols in wide-ranging ethnobotanical fieldwork is a promising approach for filling critical information gaps. Our work contributes to the Aichi Biodiversity Targets and emphasizes the need for signatory nations to the Convention on Biological Diversity to respond to these information gaps. Given our findings, we hope to stimulate the formulation of clear plans to systematically document ethnobotanical knowledge in northwestern South America and elsewhere before it vanishes.